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Biowest SAS
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Axon Medchem LLC
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GeneTex
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PAN - Biotech
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Image Search Results
Journal: Antioxidants
Article Title: Val16A SOD2 Polymorphism Promotes Epithelial–Mesenchymal Transition Antagonized by Muscadine Grape Skin Extract in Prostate Cancer Cells
doi: 10.3390/antiox10020213
Figure Lengend Snippet: EMT can be inhibited in LNCaP cells overexpressed with SOD2 SNPs. LNCaP Neo, Ala/Ala , or Val/Val were treated with 5% dextran charcoal stripped fetal bovine serum (DCC) containing ethanol (EtOH) control, 250 µM hydrogen peroxide (H 2 O 2 ), 20 µg/mL muscadine grape skin extract (MSKE), or H 2 O 2 + MSKE combination of the same concentration from day zero to day three. Subsequently, ( A ) western blot analysis of whole cell lysate was performed using EMT markers Snail, vimentin and E-cadherin. β-Actin was used as a loading control. ( B ) Quantification of western blots was performed using Image J (National Institutes of Health-NIH) normalized to β-Actin. The statistical significance of differences (one-way ANOVA and paired t-test) for Image J data was analyzed by GraphPad Prism and shows p < 0.05 (*), p < 0.01 (**), p < 0.0001 (****). Results are representative of triplicate experiments performed at least twice independently.
Article Snippet:
Techniques: Control, Concentration Assay, Western Blot